In certain circumstances rail vehicles wheels can be during operation damaged. Then, the profile of wheels is no longer circular, but it is changed depending on the type and severity of defects. When such a rail vehicle with damaged wheel operates, the quality of a ride comfort for passenger is degraded. This article is focused on the assessment of an influence of a rail vehicle running with damaged wheel on a passenger ride comfort based on results obtained from dynamic analyses. Simulations and calculations were carried out in commercial multibody software. In our research we considered one type of wheel defect the wheel-flat. This type of wheel damaging is relatively common and has such influence on ride comfort worsening, which needs to be detected and investigated.